WebJan 10, 2024 · The non-Wave makers used the BiFC co-expression assays in N. bethamiana, including ST-mRFP, mCherry-VAMP721, mCherry-SYP61, and VHAa1-mRFP, were created as described here. The coding sequences of ST 41 or VAMP721 44 were amplified and inserted into the Not I and Asc I sites of pENTR-D, followed by the LR reaction to make mRFP … WebEnter the email address you signed up with and we'll email you a reset link.
Trans-Golgi network localized small GTPase RabA1d is involved in …
WebFeb 27, 2024 · MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging. We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to Escherichia coli and altered … WebJul 21, 2016 · This was attempted by co-expressing the NAP1-XFP construct with constructs encoding various organelle markers: ST-RFP for Golgi, VAP27-1-YFP for ER-plasma membrane (PM) contact sites, VTI12-mCherry for pre-vacuolar compartments, and RabF2a-mCherry for the trans-Golgi network. fnf test playground new characters
QUIRKY regulates root epidermal cell patterning through …
WebSep 27, 2014 · The observation of the co-expression of FYVE-DsRed and mCherry-RabF2a with GFP-RabA1d revealed no significant colocalization with the late endosomal markers … WebSep 27, 2014 · The observation of the co-expression of FYVE-DsRed and mCherry-RabF2a with GFP-RabA1d revealed no significant colocalization with the late endosomal markers … WebBy co-localization with the cis-Golgi marker, GmMan1-mCherry, Rab11F-36 organelles formed tri-colored organelles, whereby Rab11F was located to the opposite 37 side of GmMan1-mCherry indicating that Rab11F-labeled structures were localized 38 ... 98 Rha1/RabF2a is on multivesicular bodies (MVBs) or pre-vacuolar compartments, fnf test playground hank