Phis2-p53

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August undefined, 2024

Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM …

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WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … daddy\u0027s noodle bar east lyme ct

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Webb上海北诺生物科技有限公司所提供的酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，质量可靠、规格齐全,上海北诺生物科技有限公司不仅具有专业的技术水平,更有良好的售后服务和优 … WebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … daddy\u0027s pit crew svg

Subcellular localization and binding elements analyses. (a) …

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

Webb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … http://www.labbase.net/Supply/SupplyItems-4229347.html daddy\u0027s noodles storrs ctWebb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … daddy\u0027s other chick baby outfit

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Phis2-p53

Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

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Webb15 nov. 2024 · To eliminate the false positive results of the TF-centered Y1H assay, the bait plasmid pGADT7-bZIP44 was verified for self-activation. pGADT7-bZIP44 + pHIS2-p53 and pGAD53m + pHIS2-p53 were respectively transformed into the Y187 yeast cells, which were spread on SD/-Trp-Leu medium and incubated at 30 ℃ for 3–5 d. Webb1 dec. 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PagWRKY75 co-transformed yeast cells were used as positive and negative control, respectively. 3.5. Generation of overexpression or knockdown plants for PagWRKY75.

Webb1 mars 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PtrHAT22 were used as the positive and negative control, respectively. 10 −1, 10 −2, and 10 −3 represent solution dilution ratio of transformed Y187 yeast cells. 4. Discussion. Webb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 …

Webb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ... Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒 …

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Webb人们最初认为p53基因是一种癌基因，但随着近十年研究的深入， p53 作为抑癌基因的功能逐渐被揭示出来。在人类50%以上的肿瘤组织中均发现了p53基因的突变，这是肿瘤中 … binson southfieldWebb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize … binson southgateWebb酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，. 产品型号：. 产品时间： 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体，毕赤酵母表达载体，毕赤 ... daddy\\u0027s partner maybe crossword cluehttp://www.coolaber.com/usr/2160/File/F/F4671.pdf daddy\u0027s of the southWebb3 mars 2024 · The pHIS2-p53/pGADT7-p53 and pHIS2-p53/pGADT7-PsnSHN2 were used as the positive and negative control, respectively. 1, 10 −2 and 10 −4 represent solution dilution ratio of transformed Y187 ... binsons royal oak faxhttp://baiyk.com.cn/ProDetail.aspx?ProID=56 binsons ostomy suppliesWebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … daddy\u0027s princess hoodie