Granuphilin c2a
WebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started! WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. Slp-4 binds vesicular Rab proteins via an N-terminal …
Granuphilin c2a
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WebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, … WebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The …
WebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures … WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of …
WebFeb 15, 2006 · Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been … Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the …
WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin …
Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its can i disembark from a cruise early nclNMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. can i dishwasher my hydro flaskWebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. fitspo of the week singaporeWebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2. 7: 24184645: 2014: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. 5: 27032672: 2016: Granuphilin exclusively mediates functional granule docking to the plasma membrane. 4: can i dishwash cutting boardWebJan 1, 2024 · The results of an in vitro binding study indicated that the C2A domain of Slp3 and Slp5 functions as an atypical Ca 2+-dependent phospholipid-binding domain ... Slp4/granuphilin was originally identified as a rabphilin-like protein associated with insulin granules in pancreatic β cells (Wang et al. 1999). can i dishwasher an electric kettleWebMar 7, 2002 · Granuphilin/Slp-4 is a member of the synaptotagmin-like protein family expressed in pancreatic β-cells and in the pituitary gland. We show by confocal microscopy that both granuphilin-a and -b colocalize with insulin-containing secretory granules positioned at the periphery of pancreatic β-cells. Overexpression of granuphilins in … can i dishwash a meat thermometerWebJan 1, 2024 · The C2A domain of Slp-4 is known to drive this protein's ability to bind cellular PMs primarily via interaction with PIP 2 (12, 13).However, the protein retains an affinity for membranes containing the anionic lipids PS and/or PI, even in the absence of PIP 2 ().Here, we set out to discern the contributions of PIP 2 and background anionic lipid binding to … can i dismantle wooden bookcase