WebDec 27, 2024 · From RELION release-3.1 onwards, partial signal subtraction after multi-body refinement is different from the one described in Subheading 3.3. Instead of using the “ relion _flex_analyse” program, the optimiser.star file from the multi-body refinement can be given directly as input to the “Particle subtraction” job-type. This new ... WebFeb 26, 2024 · Connect the map_half_A volume group to the particle subtraction job as usual. Navigate to the outputs tab of the map_half_B import job. Override the map_half_B low-level result in the particle subtraction job with the imported one (see image below) Screen Shot 2024-02-26 at 4.30.23 PM 2560×1323 408 KB. Before queueing the job, …
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Multi-body Refinement of Cryo-EM Images in RELION
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