Cisplatin zebrafish

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August undefined, 2024

WebFeb 8, 2024 · Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). In this model, … WebOct 21, 2024 · The zebrafish larvae model offers two significant advantages: (i) it can be used during a screening of novel substances and compounds against cisplatin-induced hair cell damage; (ii) it can promote a better understanding of the mechanisms related to cisplatin-induced hearing loss. 21 However, studies using zebrafish are not free of …

The identification of dual protective agents against …

WebFeb 15, 2024 · The cisplatin was used to produce a loss of CHCs to create a murine model of deafness. The AAV vectors were delivered into the scala media through the lateral wall. Compared with the control mice, the cisplatin-treated mice exhibited significantly enhanced apoptosis and autophagy in the cochleae, accompanied by a notably decreased Pou4f3 … WebJul 28, 2024 · Cisplatin is a highly effective anticancer agent that primarily functions through covalent-binding to purine bases, resulting in DNA … philhealth fee

Pou4f3 gene mutation promotes autophagy and apoptosis of …

WebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells—the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in … WebDec 1, 2024 · Zebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but … WebCisplatin-induced hair cell loss in zebrafish (Danio rerio) lateral line We have used time-lapse imaging to study cisplatin-induced hair cell death in lateral line neuromasts of zebrafish larvae in vivo. We found that cisplatin-induced hair cell death occurred much more slowly than had been shown to occur in aminoglycoside-induced hair cell death. philhealth fee 2022

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish ...

Adenosine A2A receptor blockade prevents cisplatin-induced ... - PNAS

WebFeb 6, 2014 · Zebrafish were exposed to 1000 μM cisplatin for 6 h. We, then, periodically monitored swimming depth, total swimming distance, peak swimming velocity, and mean swimming velocity of the fish for approximately 21 days. WebInner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to study drug-induced sensory hair cell death since their hair cells are similar in structure and function to those found in humans. philhealth faqsWebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells-the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in … philhealth fake id template

"WebMar 27, 2024 · Cisplatin is a platinum-based chemotherapy compound effective against a variety of cancers. However, it can cause increased reactive oxygen species (ROS) production in auditory and vestibular tissue leading to permanent hearing and balance loss. The amino acid, L-serine, has been shown to reduce ROS in some tissue types. " - Cisplatin zebrafish

Cisplatin zebrafish

Cisplatin nephrotoxicity is induced via poly(ADP-ribose) …

WebMar 6, 2013 · Cisplatin, one of the most commonly used anticancer drugs, is known to cause inner ear hair cell damage and hearing loss. Despite much investigation into mechanisms of cisplatin-induced hair cell death, little is known about the mechanism whereby cisplatin is selectively toxic to hair cells. WebJul 7, 2024 · Using cisplatin to model CICI (), we performed RNA sequencing in the mouse hippocampus to identify novel molecular contributors and found profound up-regulation of the adenosine A 2A receptor (A 2A R) by cisplatin (Fig. 1A), potentially representing a mechanism for cisplatin-induced neurotoxicity.Adenosine maintains central nervous …

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WebNov 1, 2007 · Five dpf zebrafish larvae, prelabeled with FM1-43FX were exposed to cisplatin at concentrations of 0, 0.25, 0.5, 0.75, 1, and 1.5 mM for 4 h. Doses were chosen empirically in pilot studies by identifying high doses of cisplatin that caused hair cell death after short durations without mortality of the zebrafish. WebOur findings demonstrate that cisplatin can alter the locomotion behavior of zebrafish. Behavioral changes of zebrafish according to cisplatin-induced toxicity of the balance …

WebSep 1, 2024 · Zebrafish embryos were exposed to cisplatin (0, 50, and 100 μM) for 96 h (4–100 h post-fertilization) and then stained with fluorescent dyes to reveal mitochondrial activity (rhodamine123), apoptosis (acridine orange), and oxidative stress (CellROX/MitoSOX) in ionocytes of living embryos. WebFeb 15, 2024 · Cisplatin or doxorubicin induces pyroptosis of renal tubular epithelial cells It has been reported that the typical characteristics of pyroptosis were increased LDH release, increased PI-positive...

WebMay 1, 2024 · Cisplatin is a well-known chemotherapy medication used to treat numerous cancers. However, treatment with cisplatin in cancer therapy has major side effects, such as nephrotoxic acute kidney injury. Adult vertebrate kidneys are commonly used as models of cisplatin-induced nephrotoxic acute kidney injury. WebNational Center for Biotechnology Information

WebZebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but are optically accessible, permitting observation of cisplatin injury in live intact hair cells.

WebJan 1, 2024 · This study provides evidence that cisplatin-induced hair cell loss in zebrafish is accompanied by protein nitration and Lmo4 degradation, which are emerging as … philhealth fee 2023WebNov 18, 2024 · Zebrafish have been used in many studies as an in vivo model to evaluate the cellular mechanisms of cisplatin-induced hair cell death and to identify potential … philhealth fees 2023WebDec 1, 2024 · Cisplatin ototoxicity Zebrafish Mitochondrial dysfunction Mitochondrial bioenergetics 1. Introduction Cisplatin is a chemotherapeutic agent that is commonly used in the treatment of solid tumors. philhealth field officesWebFeb 18, 2024 · For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+ B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were … philhealth fillable formWebCisplatin is a very effective anticancer drug and generates reactive oxygen species (ROS) such as superoxide anions that can deplete antioxidant protective molecules in the cochlea. ... An in vivo study of transgenic zebrafish (Brn3C: EGFP) was used to investigate the protective effects of apocynin on cisplatin-induced hair cell death. In an in ... philhealth filipiknow philhealth financial statement formWebConclusion: The zebrafish lateral line was used to identify 2 small molecules that protected against cisplatin-induced hair cell death. Key Words: CisplatinVHair cellVOtotoxicityV Protection. Otol Neurotol 36:519Y525, 2015. Cisplatin is a commonly used anticancer drug in the treatmentofmanycancers,includinglungcancer,ovarian philhealth fill up form