WebDec 24, 2015 · The precipitate was washed six times with extraction buffer and eluted in 2× Laemmli buffer. Anti-GFP Trap-A beads (gta-10; Chromotek) were used for IP. For protein immunoblots, proteins were separated by 12% SDS-PAGE and transferred to polyvinylidene fluoride membrane (Roth) by semidry blotting in 25 mM Tris-HCl, pH 8.3, 150 mM … WebThe ChromoTek GFP-Trap® is a ready-to-use affinity resin for immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap consists of an anti-GFP Nanobody/ V H H coupled to agarose beads, magnetic agarose beads, magnetic particles or multiwell plates. Specificity. GFP, EGFP, CFP, YFP, BFP and many more GFP derivatives, see: Fluorescent protein ...
GFP Nanobody Magnetic Agarose, ChromoTek GFP-Trap® gtma …
WebHello, you can melt Chromotek GFP-TRAP at 95*С and use magnet to remove magnetic agarose beads as you described without problem. Cite 3 Recommendations 21st Jan, 2024 Adam Thiesen Massachusetts... WebGFP-Trap是ChromoTek旗下Nano-Traps系列最具代表產品之一。 Trap 是 陷阱、捕捉器的意思，顧名思義GFP-Trap就是捕捉GFP（或攜帶GFP Tag的fusion protein）的一種技術。 其原理是通過將anti-GFP的羊駝奈米抗體，與固相載體（agarose beads, magnetic agarose beads, Dynabeads or multiwell plates等）直接共價連接，形成一個高效能捕捉GFP的工 … slow cooker ranch chicken thighs
GFP-Trap for immunoprecipitation Proteintech Group
Web1 day ago · The cleared lysate was then incubated with GFP-trap beads (Chromotek, gtma) or incubated with anti-HA tag beads (Thermo, 88836) overnight at 4 °C. The beads were then washed 3 times with wash ... WebThese reagents allow your GFP-fusions to be perfect candidates for immunoprecipitations, Co-IP, mass spectroscopy, enzyme activity measurements, and ChIP analysis. GFP-Trap Affinity Reagents are perfect for fast, clean, and efficient one-step immuno-purification of these fluorescent fusion proteins and their interacting factors. WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg-Martinsried Germany phone: +49 89 124 148 80 fax: +49 89 124 148 811 ChromoTek Inc. 62-64 Enter Lane Islandia, NY 11749 USA phone: 631 501 1058 fax: 631 501 1060 … slow cooker raspberry jam